Cytochrome C Reductase Activity Assay (TBS2116)

Description: This kit is designed to measure the NADPH cytochrome C reductase activity in biological samples including cell and tissue extracts, and in purified microsomes (endoplasmic reticulum, ER). Eukaryotic NADPH-Cytochrome C reductase (NADPH cytochrome P450 reductase, EC 1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which is the cytochrome P450 family of enzymes, responsible for xenobiotic metabolism.NADPH-cytochrome C reductase is widely used as an endoplasmic reticulum marker and as a biomarker of ecological pollution and dietary lipid uptake. This kit is based on a colorimetric assay that measures the reduction of cytochrome C by NADPH-Cytochrome C reductase in the presence of NADPH. The reduction of cytochrome C results in the formation of distinct bands in the absorption spectrum and the increase in absorbance at 550 nm is measured with time. ApplicationsMeasuring NADPH-cytochrome c reductase levels in biological samples after exposure to drugs or other xenobiotics.Detection of ER during isolation and subcellular fractionation by density gradient separation. Key FeaturesFlexible: The kit can be used for 96-well plates or 384-well plates.Accurate: Accurate detection of cytochrome C reductase activity in small size as 50 µL samples.Simple: Just load-Read. The kit can be used for a robust method.Time-saving: Less than 30 minutes. Kit ContentsAssay Buffer15 mLCytochrome C1.2 mLNADPH2 mLCytochrome C Reductase Positive0.25 mLCytochrome C Oxidase Inhibitor1 mL Storage ConditionsStore kit at -20°C. Shelf life of 2 years. Protect from light.Ship with blue ice.

Price: 546 USD

Location: Sunnyvale, California

End Time: 2025-01-14T22:48:01.000Z

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