Structure and Function of Membrane Proteins by Ingeborg Schmidt-Krey (English) H

Description: Structure and Function of Membrane Proteins by Ingeborg Schmidt-Krey, James C. Gumbart This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to furtherunderstand these essential proteins and their many biological roles. Back Cover This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles. Table of Contents Expression and Purification of Human Mitochondrial Intramembrane Protease PARL.- Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies.- Biochemical Characterization of GPCR–G Protein Complex Formation.- Electrophysiological Approaches for the Study of Ion Channel Function.- Isothermal Titration Calorimetry of Membrane Proteins.- Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level.- Structure Determination of Membrane Proteins Using X-Ray Crystallography.- Studying Membrane Protein Structures by MicroED.- Single-Particle Cryo-EM of Membrane Proteins.- Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy.- NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel.- Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering.- Preparing MembraneProteins for Simulation Using CHARMM-GUI.- Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide.- Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins.- Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions.- Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations.- Large Scale Molecular Dynamics Simulations of Cellular Compartments. Feature Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts Details ISBN1071613936 Series Methods in Molecular Biology Language English Year 2021 ISBN-10 1071613936 ISBN-13 9781071613931 Format Hardcover Series Number 2302 DOI 10.1007/978-1-0716-1394-8 Publisher Springer-Verlag New York Inc. Edition 1st Imprint Springer-Verlag New York Inc. Place of Publication New York, NY Country of Publication United States Pages 358 Publication Date 2021-04-21 AU Release Date 2021-04-21 NZ Release Date 2021-04-21 US Release Date 2021-04-21 UK Release Date 2021-04-21 Illustrations 70 Illustrations, color; 27 Illustrations, black and white; XII, 358 p. 97 illus., 70 illus. in color. Author James C. Gumbart Edited by James C. Gumbart Edition Description 1st ed. 2021 Alternative 9781071613962 DEWEY 572.696 Audience Professional & Vocational We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:138873722;

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